فهرست مطالب

مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
سال پنجاه و یکم شماره 2 (پیاپی 100، تابستان 1387)

تاریخ انتشار: 1387/06/20
تعداد عناوین: 11

فارسی
English

انتخاب همه

Identification of Leishmania Species Causing Cutaneous Leishmaniasis by RAPD-PCR

Masoud Mohajery, Homa Hajjaran, Ali Akbar Shamsiyan, Jalil Tavakkol Afshari Fatemeh Saadabadi Pages 79-86

Introduction
Leishmaniasis, especially cutaneous leishmaniasis is considered as an important health problem in many parts of Iran such as Neishabour, in Razavi Khorasan Province. Since DNA of every parasite such as every organism is specific, this facilitates extensive use of DNA for diagnosis and identification of parasite species. Among these methods, RAPD-PCR seems to be a useful method. Since Neishabour is an important focus for cutaneous leishmaniasis and so far molecular researches haven’t been done, this study was done to identify different species of leishmania parasites causing cutaneous leishmaniasis by RAPD-PCR.
Materials And Methods
In this study a total of fifty-seven patients, whom their disease confirmed by direct smear, were recruited and samples isolated and cultured in NNN medium, followed by sub-culturing in RPMI-1640. This study was approved by the local ethics. Then DNA was extracted by proteinase k and desirable samples were amplified by RAPD-PCR method, using four oligoprimers. Electrophoresis patterns from each isolate were compared with the reference strains of L.major, L. tropica and the marker.
Results
The results of this study indicated that L. tropica is the responsible parasite for causing cutaneous leishmaniasis, in Neishabour, Northeast of Iran.
Conclusion
It seems that Leishmania tropica is the only causative agent of Cutaneous Leishmaniasis in this study area. RAPD-PCR technique is a suitable tools for Leishmania characterization the in epidemiological studies.
Keywords: Leishmania major, Leishmania tropica, RAPD, PCRIran

Abstract View Paper Original: Persian
Serologic Evaluation of HIV-1 and Syphilis in Patients withClinical Anogenital Wart

Masoud Maleki, Zari Javidi, Vahid Mashayeki, Naser Tayebi Meybodi, Yalda Nahidi, Habibollah Esmaeili, Leily Hafizi Pages 87-94

Introduction
Genital infection with human papilloma virus (HPV) is the most common sexually transmitted disease in the United States. Patients having genital warts are at excessive risk of HIV and syphilis. Uninformed patients with high risk behaviors can help spread this condition in society. Therefore, CDC recommends HIV and syphilis screening for those with a sexual transmitted disease or a sexual risk factor. But this strategy is rarely considered in Iran. The aim of this study was to evaluate the cost-effectiveness of HIV and syphilis screening tests in patients with genital wart.
Materials And Methods
In this case-control study, 100 patients with genital wart referring to Dermatology Department of Imam-Reza Hospital (August 2005 - August 2007) were included as the case group. This study was approved by the local ethics. The Control group was the same in number, age average, sex and marital status. All 200 patients studied considering HIV or syphilis infection. Finally, collected data were statistically analyzed by SPSS software, using χ2and student T test.
Results
Male to female ratio was 0.87. Majority of patients aged 30-39 years. Two patients were HIV positive and only one had Syphilis, without significant difference with the control group (Pv =0.29, Pv =0.50).
Conclusion
Screening is not cost effective in patients with asymptomatic anogenital warts. Since both HIV positive cases were intravenous drug abusers, this study emphasized on HIV and syphilis screening in patients with high risk behavior.
Keywords: Anogenital wart, Condyloma acuminatum, Sexually transmitted infections, Syphilis, Screening, HIV

Abstract View Paper Original: Persian
Frequency of Dermatophytoses

Seyyed Ali Akbar Shamsian, Mohammad Javad Yazdan Panah, Monireh Mokhtari Amir Majdi, Mahdi Moradi Marjaneh, Sara Saboori Rad Pages 95-102

Dermatophytosis is a fungal infection caused by dermatophytes, which have the ability to invade keratinized tissues such as hair, nail and skin. Epidemiology of the disease depends on age as well as prevailing hygiene, socioeconomic status and cultural conditions.
Materials And Methods
This descriptive study was conducted from March 2006 to March 2007 at Mashhad University of Medical Sciences, on 599 patients with suspected cutaneous fungal infection at Dermatology Clinic of Ghaem Hospital Mashhad University of Medical Sciences, Mashhad, Iran. Direct examination of smears and culture on SCC were used to diagnose the infection in mycology labs of Ghaem Hospital and Mashhad branch of ACECR.
Results
Overall, 384 patients had fungal infection (64.1%), out of which 137 cases were suffering from dermatophytosis. Numbers of male and female cases were 91 and 46 respectively (p= 0.002). Maximum infection rate found in 21-30 years age group and tinea corporis was the most common dermatophytic infection (56.2%) followed by tinea cruris (20.4%) and tinea pedis (13.8%). The frequencies of etiological agents isolated from patients were as follows: Tricophyton rubrum, 44.5%; Epidermophyton floccosum, 21.9%; Tricophyton mentagrophyte, 11%; Tricophyton verrucosum, 7.3% and Microsporum canis, 7.3%.
Conclusion
Since high prevalence of dermatophytosis reported in this study, public education on the potential risk factors, diagnosis and treatment of the disease are recommended and more studies to determine the main agents of dermatophytosis in other regions of our country are necessary.
Keywords: Dermatophytosis, Dermatophyte, Epidemiology, Tinea

Abstract View Paper Original: Persian
Evaluation of Serum Zinc and Copper Concentrations in Epileptic Patients

Habibollah Nemati Karimuy, Musareza Hajzadeh, Zahra Daei Pages 103-108

Discovering ethiology of epilepsy is inevitable to find effective treatment methods. The aim of this study was to evaluate serum zinc and copper concentrations in patients with epilepsy.
Materials And Methods
This study was done in 2006. A total ofsixty patients (25 males, 35 females; mean age 22.43±1.18 years) with epilepsy and 60 age-matched healthy subjects (25 males, 35 females; mean age 25.27±1.091 years) enrolled in this study. This study was approved by the local ethics. Serum concentrations of Zn and Cu assessed for all subjects in both groups with an atomic absorption spectrophotometer. Results were demonstrated as Mean± SEM for Cu (μg/lit) and for Zn (μg/dl). The results compared by unpaired t-test and when p<0.05, the differences considered to be significant.
Results
Mean copper level was 947.98±30.39 μg/lit and 967.41±28.25 μg/lit in the case and control group, respectively. The serum concentration of zinc was 86.81±1.55 μg/dl in patients and 94.48 ± 11.11 in the control group μg/dl. The differences between the two groups was insignificant (p>0.05).
Conclusion
In contrast with some other studies which showed a difference in serum concentrations of Cu and Zn in epileptic patients, our data demonstrated that concentrations of serum zinc and copper in epileptic patients are not statistically different from the control group. Therefore, it is suggested that serum levels of Zn and Cu are not the strong determinant factors involved in inducing epilepsy; thus, it can be concluded that epileptic episodes in human may not be related to the serum levels of both trace elements Cu and Zn.
Keywords: Copper, Epilepsy, Zinc

Abstract View Paper Original: Persian
Step by step clinical diagnosis

Mahmoud Farhoudi, Ali Mokhtarifar, Saadat Mirsadraee, Behzad Ramezani Farkhani Pages 137-143

Huge splenomegaly has a large list of differential diagnosis (DDx) with infectious disease, Liver disease and cancers that we rolled out these DDx with step by step diagnosis. And finally we found hereditary Gucher’s disease, in spite of his age (49yrs). By the recurrent hemorrhage and severity of thrombocytopenia and leucopenia, spleenectomy was done and the patient discharged with good general appearance.Gaucher’s disease should be considered in the differential diagnosis of patients with unexplained organomegaly, easy bruisability and/or bone pain. Bone marrow examination usually reveals the presence of Gaucher cells; however all suspected diagnoses should be confirmed by demonstrating deficient acid b-glucosidase activity in isolated leucocytes or cultured fibroblasts.
Keywords: Splenomegaly, Epistaxis, Storage disease, Gaucher's disease, Thrombocytopenia

Abstract View Paper Original: Persian
Human Papillomaviruses, HIV-1 and Syphilis

Mahmoud Panahi Pages 144-148

Introduction
There are a variety of proliferative squamous lesions of the skin in human papilloma viruses infection. Oral cavity and anogenital tract are the mucosal surfaces that usually become infected. New technology on recombinant DNA assays makes researchers able to diagnose suspected lesions of the skin by viral DNA extraction. Mucocutaneous HPVs are generally associated with warts.Sexually active persons with high rate of sexually transmitted infections (STI) and immunosuppressed patients like kidney receivers and pregnant women are candidates of HPV (wart) infections. The high incidence of wart virus infection in association of other STI suggests that anogenital warts are transmissable and in 2/3 of cases it appears through sexual contact. Anogenital warts are more common in women than men.Types of 6 and 11 of HPV usually produce warts but rarely may they be cancerogenic. However HPV types 16-18-31-33-35 are more cancerogenic, and occasionally may be responsible for the skin prolifeative squamous lesions (warts).In this issue of journal, Maleki M.D, et al report on a study of seroepidemiology of papilloma virus infection in HIV-1/AIDS and syphilitic patients. The selection of the target population for sexually transmitted infection (STI) and methodology of survey are correct and having a control group makes the study acceptable. Although there is equality of HIV-1 and syphilitic patients, two groups, challenging besides each other, but, patients in both groups are IV drug abusers. This is an important risk factor for HIV-infection, and so, the role of papilloma Virus infection becomes less important. In this study the diagnosis of warts made only by the clinical appearance. It is necessary to know, though clinical diagnosis of wart is easy, but in some cases PCR-DNA assay is essential and confirmative.All together the present work is the first extended study of papilloma anogenital wart in the region and this can be the beginning of large multicenter survey in the future.

Abstract View Paper Original: Persian

علمی مصوب

مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد

Medical Journal of Mashhad University of Medical Sciences

دوماهنامه پزشکی

آخرین شماره | آرشیو

ISSN: 1735-4013

صاحب امتیاز:

دانشگاه علوم پزشکی مشهد

مدیر مسئول:

دکتر عبدالله بهرامی

سردبیر:

دکتر غلامحسین کاظم زاده

تلفن نشریه: ۰۵۱-۳۸۵۳۳۶۳۰

اطلاعات بیشتر نشریه

درباره نشریه پیام به نشریه سایت اختصاصی نشریه پذیرش الکترونیکی مقاله

به جمع مشترکان مگیران بپیوندید!

فهرست مطالب

مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد سال پنجاه و یکم شماره 2 (پیاپی 100، تابستان 1387)

مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد

Medical Journal of Mashhad University of Medical Sciences

مجله دانشکده پزشکی دانشگاه علوم پزشکی مشهد
سال پنجاه و یکم شماره 2 (پیاپی 100، تابستان 1387)