mohammad seyedabadi

Testicular torsion is a critical urological emergency that can lead to testicular ischemia and significant tissue damage. Citrulline, a supplement known for enhancing cellular metabolism and mitigating oxidative stress and inflammation, has been explored for its protective effects against testicular injury resulting from torsion and detorsion in rat models.

This study involved 42 Wistar rats, divided into six groups: Sham, torsion/detorsion (T/D), and four groups receiving varying doses of Citrulline (300, 600, 900 mg/kg) and vitamin E (20 mg/kg). A surgical procedure was performed to induce torsion by rotating the left testicle for 4 hr, followed by reperfusion. Daily oral administration of the supplements continued for one week post-surgery. Assessments included oxidative stress markers, apoptosis, inflammation, pathology, and sperm parameters. Statistical analysis was conducted using GraphPad Prism.

Citrulline administration at doses of 600 and 900 mg/kg significantly reduced malondialdehyde (MDA) and reactive oxygen species (ROS) levels. Additionally, it increased glutathione (GSH) levels and decreased protein carbonyl levels at the 900 mg/kg dose. The expression of interleukin-6 (IL-6) decreased at 900 mg/ kg, tumor necrosis factor-alpha (TNF-α) levels dropped at 600 and 900 mg/kg, and the pro-apoptotic factor Bax was reduced at all doses. Sperm analysis showed improved sperm count and motility at the 900 mg/kg dose. Histological examination revealed significant positive effects of Citrulline on testicular tissue.

Citrulline effectively lowers oxidative stress, inflammation, while enhancing sperm quality and pathological outcomes. These results indicate that Citrulline has potential as a therapeutic agent for testicular torsion.

Exposure to noise leads to the production of reactive oxygen species (ROS) or free radicals in the brain. Even a slight increase in free radical levels in brain cells can disturb the central nervous system's structure and contribute to related diseases, including Alzheimer's disease. Therefore, this study aimed to investigate the protective effects of four weeks of regular aerobic training and benfotiamine supplementation on noise-induced oxidative stress in the brains of mice.

Forty male mice were randomly divided into five groups: a control group, a noise-exposed group subjected to occupational noise (100 dB, 4 hours per day), a noise+exercise group, a noise+benfotiamine group, and a noise+exercise+benfotiamine group. All animals, except those in the control group, were exposed to daily occupational noise emitted from a loudspeaker. After noise exposure, benfotiamine (200 mg/kg) was administered via gavage to two groups of mice. The training groups then performed aerobic exercise on a treadmill (5 days per week, 30 minutes per day at 12 m/min) according to protocol. All interventions were performed for four consecutive weeks. Seventy-two hours after the last exercise session, the animals were anesthetized, and brain tissues were immediately collected and homogenized for further evaluation. Brain tissue levels of ROS, malondialdehyde, protein carbonyl, reduced glutathione, and total antioxidant capacity—markers of oxidative stress—were measured using biochemical methods. One-way analysis of variance and Tukey's post hoc tests were used to analyze the data.

The results showed that, compared to the control group, daily exposure to occupational noise for four hours significantly increased levels of ROS, malondialdehyde, and protein carbonyl (P<0.001) and significantly decreased antioxidant capacity (P<0.01) and glutathione levels (P<0.001) in brain tissue. Four weeks of treadmill exercise reduced ROS levels (P<0.01) compared to the noise group but did not significantly impact other oxidative markers in the brains of animals exposed to daily noise (P>0.05). Daily administration of benfotiamine supplements led to reductions in ROS and malondialdehyde levels and an increase in reduced glutathione in brain tissues of noise-exposed mice compared to the noise group (P<0.05). However, this supplementation did not significantly affect protein carbonyl levels or total antioxidant capacity (P>0.05). Compared to the noise group, combining aerobic exercise with benfotiamine consumption significantly reduced ROS and protein carbonyl levels and increased glutathione in the brain tissue of noise-exposed animals (P<0.001). However, this combination had no significant effect on malondialdehyde levels or antioxidant capacity (P>0.05).

The results of this study indicate that benfotiamine, as an effective antioxidant, may have a protective and preventive role in reducing oxidative stress in brain tissue caused by occupational noise in mice. Daily benfotiamine supplementation combined with aerobic exercise may be an effective strategy for preventing oxidative disorders related to occupational noise exposure by inhibiting ROS production and improving certain oxidative stress markers. Further studies are needed to clarify the mechanisms underlying the combined effects of benfotiamine and aerobic exercise in reducing noise-induced oxidative stress.

Poisoning is a significant public health concern, encompassing a broad range of sudden and severe health issues resulting from the ingestion, inhalation, or contact with toxic substances. The potential for injury or fatality necessitates urgent medical attention and care. With the ongoing advancements in artificial intelligence (AI) and its increasing integration into medical and pharmaceutical domains, there is a growing interest in exploring the role of AI in the context of poisonings. This study aims to investigate the potential applications of AI in the management and treatment of poisoning.

This research is a review by searching the keywords ("Artificial intelligence") [TIAB] AND (poisoning [TIAB] OR toxicity[TIAB] OR intoxication[TIAB] OR Toxin[TIAB] OR poison[TIAB]) was searched in the internet databases PubMed, Scopus, and Google Scholar search engine in 2016-2024.

Enhancing system management and treatment approaches is crucial in preventing accidental and intentional poisoning. This can be achieved by incorporating cutting-edge medical equipment, such as those equipped with AI. AI technology can recognize complex patterns beyond predefined rules and process large amounts of data, exceeding human capabilities. In the future, more progress in AI will likely affect various areas of healthcare, including poison prevention and treatment, to improve patient outcomes and reduce the burden of poisoning on healthcare systems.

Perfluorooctanoic acid (PFOA) is a persistent organic pollutant (POP), broadly present in the environment. Due to long biological half-life, it is accumulated in the body, especially the liver, causing hepatocellular damage. This study was designed to assess the effects of rutin on PFOA-induced liver damage in rats. Male Wistar rats were exposed to PFOA (10 mg/kg/day) alone, or in combination with different doses of rutin (25, 50, and 100 mg/kg/day) by oral gavage for 4 weeks.  PFOA altered the levels of liver enzymes, induced a notable change in the tissue structure of the liver, caused some levels of mitochondrial dysfunction, and increased the expression of pro-apoptotic and pro-inflammatory genes. Co-treatment with rutin mitigated the PFOA-induced elevation of liver enzymes, histopathological defects, oxidative damage, and mitochondrial dysfunction. In addition, rutin declined the stimulatory effects of PFOA on the Bax: Bcl2 ratio and reduced the PFOA-induced gene expression of TNF-α, IL-6, NF-ƙB, and JNK. These findings suggest rutin as a protective agent for PFOA-induced liver injury, albeit the protection was partial. Possible mechanisms are inhibition of oxidative stress, mitochondrial dysfunction, and inflammatory response.

Given the importance of differential diagnosis of different papillary urothelial cancers, expression, intensity and distribution of p53 were evaluated in different bladder cancers using immunohistochemistry.

In a retrospective study, archived samples of patients with different papillary urothelial cancers in Shohaday-e-Khalij Fars Hospital were selected. The samples were grouped according to the reported diagnosis into papilloma, low-malignant potential papillary tumors, low-grade papillary carcinoma, and high-grade papillary carcinoma. The samples were then immunohistochemically stained for p53. 

The majority of patients with papillary urothelial carcinoma were positive for p53. However, they were different in terms of intensity (p<0.001) and distribution (p<0.01). Low-malignant potential and low-grade malignant samples demonstrated low levels of p53 expression, whereas high-grade malignant samples exhibited high levels of its expression. Comparison of p53 distribution revealed focal expression in low-malignant potential group and diffused expression in malignant groups.

The results of this study suggest p53 expression, intensity and distribution as a useful marker for differential diagnosis and therapeutic management of different urothelial cancers.

Neuroprotective effects of L-carnitine on damage caused by fetal exposure to ethanol have been demonstrated. The purpose of this study was to evaluate the effects of different doses of L-carnitine at different stages of pregnancy on the memory of two month old offsprings whose mothers consumed alcohol during pregnancy.

For the purpose of this study, 55 pregnant female rats were randomly divided into 11 groups of 5, including control group, which received drinking water, and the ethanol group which received drinking water containing 10 % (w/v) ethanol. L-Carnitine treated group received L-carnitine in doses of 50, 100, or 150 mg/kg in addition to the ethanol, in the first 10 days or the second 10 days of pregnancy. For modeling the third trimester of pregnancy, doses of L-carnitine were gavaged to the rat neonates for 10 days. After two months of birth, the memories of offsprings were evaluated using Passive Avoidance Task (PAT) and Novel Object Recognition Test (ORT).

Administration of L-carnitine significantly increased discrimination of the ORT index and the escape latency of entry to dark compartment in PAT by two month old offspring compared to the ethanol group. The mentioned indices were significantly increased at a dose of 150 mg/kg compared to the dose of 50 mg/kg throughout all pregnancy periods. These indices were significantly different when l-carnitine was administered in the second 10 days of pregnancy compared to any other periods.

The administration of L-carnitine during pregnancy can prevent alcohol-induced memory impairment in rat offsprings. This effect was more significant at higher doses of L-carnitine. The best response was seen when L-carnitine was administered to mothers in the second 10 days of pregnancy.

Olanzapine (2-methyl-4-(4-methylpiperazin-1-yl)-10H-thieno [2, 3-b][1, 5]benzodiazepine) is an antipsychotic drug with poor water-solubility that has a suitable affinity to some receptors. This benzodiazepine derivative is a new (atypical) antipsychotic drug and has a wide range of efficacy, especially in treatment of patients with negative psychiatric symptoms of schizophrenia. Olanzapine is used in psychotic disorders, mood disorders, and acute restlessness in bipolar patients. Its oral absorption is low and about 40% of the drug is metabolized in first-pass hepatic metabolism. This drug has low permeability into the brain related to remove by p-glycoprotein efflux and blood-brain-barrier (BBB) existence. Emerging nanotechnology during recent years demonstrated the tremendous ability of nanoparticles as versatile nanocarrier systems. This emerging technology has raised promising attempts to address the significant impact on decreasing side effects of drugs and their associated defects. Olanzapine can be administrated by different routes. Intranasal delivery of olanzapine to the brain due to lack of blood-brain-barrier in the olfactory system has facilitated its delivery. In this review article, several olanzapine nano-drug delivery systems are listed by reviewing their results, including nanosuspensions, nanoemulsions, solid lipid nanoparticles (SLN), niosomes, transfersomes, nanostructured lipid carriers (NLC), polymeric nanoparticles (PNP), etc.

Cognitive impairment is an unpleasant and progressive mental disorder characterized by learning and memory disabilities. Stress and alcohol are two known environmental factors that increase cognitive impairment. This study was designed to evaluate the relative role of cyclooxygenase 2 in alcohol or stress‑induced cognitive impairment.

Male Wistar rats were randomly divided into groups with six rats in each. The groups included sham, control, alcohol (15% ethanol in drinking water), and restraint stress (restraint 6 h per day). Three separated groups received celecoxib at a dose of 20 mg/kg in addition to those listed above. The treatments continued daily for 28 days. The object recognition task (ORT) and Morris water maze (MWM) are used to evaluate the learning and memory.

Alcohol or restrain stress significantly increased the time and distance needed to find the hidden platform in MWM. Furthermore, they decreased the recognition index in ORT compared to the control group. Administration of celecoxib significantly decreased the required time and traveled distance to reach the platform in alcohol‑treated animals but not in the stress‑exposed rats. Celecoxib also significantly increased the recognition index both in alcohol‑ or restraint stress‑exposed animals.

We found that either alcohol or restraint stress impairs memory in rats. In MWM, celecoxib improved the alcohol‑induced memory impairment but could not show a reduction in memory deterioration due to restraint stress. In ORT, celecoxib reversed memory impairment due to both alcohol and restraint stress.

Recurrent aphthous stomatitis (RAS) is the most common painful ulcerative disease of oral mucosa happening in ~20% of people. Aimed to develop Myrtus communis L. (Myrtle) containing oral patches, we applied box-behnken design to evaluate the effect of polymers such as Polyvinyl pyrrolidone (PVP), Gelatin, Methylcellulose (MC) and Pectin.
The patches properties such as tensile strength, folding endurance, swelling index, thickness, mucoadhesive strength and the pattern of myrtle release were evaluated as dependent variables. Then, the model was adjusted according to the best fitted equation with box behnken design.
The results indicated that preparation of myrtle patch with hydrophilic polymers showed the disintegration time up to 24h and more. Using of polyvinyl pyrrolidone as a water soluble polymer and a pore-former polymer led to faster release of soluble materials from the patch to 29 (min-1). Also it decreases swelling index by increasing the patch disintegration. Gelatin and Pectin, with rigid matrix and water interaction properties, decreased the swelling ratio. Pectin increased the tensile strength, but gelatin produced an opposite effect. Thinner Myrtle patch (about 28µm) was obtained by formulation of methyl cellulose with equal ratio with polyvinyl pyrrolidone or gelatin.
Altogether, the analysis showed that the optimal formulation was achieved with of 35.04 mg of Gelatin, 7.22 mg of Pectin, 7.20 mg of polyvinyl pyrrolidone, 50.52 mg of methyl cellulose and 20 mg of Myrtle extract.

The optimal imaging time of a radionuclide scintigraphy is the time at which the organ of interest has the maximum uptake of the injected radionuclide. This study was performed to investigate the maximum uptake time of 99mTc-DMSA in rat renal scan.
Renal scintigraphy was performed with 3 mCi of 99mTc-DMSA. Planar images were acquired every 20 minutes for 8 hours post-injection using a small-animal SPECT.
Activity and the count rate per pixel (CRPP) of the kidneys peaked 1 h post-injection, plateaued for about 1 h, and declined time-dependently. Kidney to background ratio (KBR) reached to 61.7% at 1 h after injection and remained almost constant afterwards.
The kidneys had maximum emission and CRPP between 1 to 2 h after 99mTc-DMSA injection, whereas there was no significant difference between the KBRs after 1 h. Our results showed that image acquisition of 1-2 h post-injection is recommended for renal scintigraphy with DMSA in rat.

Toxic and direct teratogenic potential of two dominant Iranian cultivars of Carthamus tinctorius (safflower), floret extracts, IL 111 and LRV 51 51, were investigated. The extracts are commonly used in foods and medicinal products. Neither death nor alteration of stereotype activities was observed with IL 111 and LRV 51 51 extracts up to 17 g/kg in 48 h in mice and rats. Haemoglobin was decreased, prothrombin time was prolonged, serum TG, LDH, CPK and cholesterol was increased in rats which received IL 111 (1 g/kg) for 9 weeks (p50: 78 and 106 µg/ml, respectively). The total number of differentiated (stained) limb bud foci was also decreased when cells were exposed to either of C. tinctorius extracts (p<0.01). When the cytotoxicity was taken into account, the ratio of Alcian stained differentiated cells to the cytotoxicity was not significantly altered with any of the extracts, suggesting no remarkable level of teratogenicity.