Effect of Aloe Vera Extract on Testicular Tissue of Embryo of Diabetic Rats

Testis is an important organ of the male reproductive system and its structure could be influenced by diabetes. Aloe Vera has a hypoglycemic effect; thus, the present study evaluates the effect of Aloe Vera on the testis tissue of a 20-day embryo of rat born from a diabetic mother. Thirty female Sprague Dawley rats were randomly divided into three groups, subsequently; two groups were injected with streptozotocin (50 mg/kg/IP) and the males were placed next to female rats in a separate cage in order to mate. One group of rats received aloe vera extract (400 mg/kg), orally, in gestational age during while others were giving distilled water. After 20 days, rats were sacrificed and their embryos were removed and fixed by 10% formalin. The embryos were processed and embeded in paraffin. Five µm sections were made and stained by hematoxyline- eosin and cellular alteration of testis was evaluated. Data were analyzed using one-way ANOVA. Mean of body weight, seminiferous tubules diameter, spermatogonia numbers, leydig cells and sertoli cells were significantly different in all groups (p<0.05). The present study demonstrated that Aloe Vera extract can increase the spermatogonium, Leydig and Sertoli cells in diabetic rats.