Effect of static magnetic field on apoptosis induction and altering the cell cycle of T-lymphoblastoid cells

Apoptosis or programmed cell death is a normal physiological process of cell that leads to active and natural development and maintenance of tissue homeostasis. Apoptosis occurs due to different external factors. This study aimed to investigate a low risk method to induce apoptosis in human leukemia cells using static magnetic field (SMF). In this laboratory research، the cultured human T-lymphoblastoid cell line (Jurkat E6. 1)، exposed to 6mT SMF for 6 and 24 hours. Luminometry، flowcytometry and western blot were used to measure total apoptosis rate، primary apoptosis rate and phosphorylated ATM and E2F1 proteins، respectively. Increasing in the rate of apoptosis in treated cells according to control cells was significant 36 hours after treatment with 6mT SMF for 6 (p=0. 011) and 24 (p=0. 055) hours. 36 hours after treatment with 6mT SMF، the first significant difference was seen between treated and control cells (p<0. 001). 24 hours after cells exposure to 6mT SMF، cells population was decreased in G2 and S and 36 hours after treatment، the population was increased in G2 and S and decreased in G1. Exposure to 6mT SMF increased the amount of phosphorylated ATM protein in 1981 Serin and E2F1 protein in 31 Serin positions. 6mT SMF induces apoptosis in exposed Jurkat cells by enhancing and activating ATM and E2F1 proteins.