Isolation and determination antimicrobial susceptibility pattern of Klebsiella rhinoscleromatis strains isolated from consumed powdered infant formula milk in NICU ward

K. pneumonia subsp.rhinoscleromatisis a non-motile gram negative coccobacillus, in the Enterobacteriaceae family. This organism is found in the environment and is considered to be part of the normal flora of the skin, respiratory and gastrointestinal tracts of humans and animals. Milk and its products might represent important sources of pathogenic Klebsiella species. The goal of this investigation was isolation and determination of antimicrobial susceptibility pattern of K. pneumonia subsp.rhinoscleromatis strains isolated from consumed powdered infant formula milk in NICU ward. In this cross-sectional study, total 125 consumed powdered infant formula milk in NICU ward were surveyed for microbial contamination. FDA protocol was used for Isolation and Identification of K. pneumoniaesubsp.Rhinoscleromatis from samples. Antimicrobial susceptibility test was carried out based on CLSI (2011) recommendations by using the standard disc diffusion method. In this study, K. pneumonia subsp.rhinoscleromatis was isolated from 3 (2.4%) of 125 powdered infant formula milk samples. Antimicrobial susceptibility results showed that all isolated strains are sensitive (100%) to amikacin, cefotaxime, nalidixic acid, chloramphenicol, ciprofloxacin, ticarcillin and minocycline. awareness of drug resistant Klebsiella spp. is important for the food industry. In the absence of control measures, drug resistant organisms might be transmitted to humans by the consumption of milk, powdered infant formula milk and milk products.