Association of CCKAR Gene Polymorphism with Gallstone Disease

Backgrounds and Bile stagnation leads to cholesterol concentrates and Crystal formation that are gall stone precursors. Cholecystokinin kinase hormone receptor (CCKAR) role in contraction and depletion of gall bladder. Polymorphisms of this receptor could have some role in gall bladder stone formation. Study on 116 gallstone cases and 118 healthy controls were performed. For molecular analysis، amplification of polymorphism containing sequence was obtained by polymerase chain reaction and then genotyping were carried out by restriction fragment length polymorphism. Finally statistical analysis had carried out for detection of association. With case-control study we recognize that C/T genotype in rs1800857 polymorphism increases gall stone risk more than 3 times (P=0/035; OR=2/954; CI95%= 1/072-8/14). This analysis confirms previous studies on rs1800857 polymorphism susceptibility to gall stone only in female and clarify the importance of this region to make a disease prediction.