Evaluation of Effects of Angi- Pars on Kidney, Brain and Liver Tissues of Chronic Diabetic Rats

Diabetes mellitus (DM) is defined as a disorder in which the prominent role of oxidative stress is well documented. Treatment of (DM) with herbal medicine products is one of the main focuses of investigators and to date several reports are published regarding properties of herbal plants. Regardingly، several types of plants are proposed for treatment of (DM). Thus، the current investigation is designed to explore therapeutic properties of Angipars as an herbal plant on tissue injuries associated with (DM) in rats. This experimental study was performed on 48 male wistar rats weighting 200-250 grams. The induction of DM was carried out by intra- muscular injection of 50mg/kg of STZ (streptozotocin) in a region between the two ears of animal، Approximately، 56 days following the induction of DM rats were divided into four main groups (12 rats in each group): first group received normal saline for 14 consecutive days and three another groups were injected with Angipars، insulin and Angipars-insulin as the same duration respectively. 24 hours after the last injection، animals were humanely killed and brain، liver and kidney tissues were analyzed regarding tissue injuries by histo-chemistry analysis. Our results showed that all of the studied animals had tissue injuries while significant decreased injuries were observed in Angipars-insulin treated group. In the majority of microscopic fields of kidney of untreated rats red blood cells were observed in tubular tissues of kidneys of animals. Likewise، kidney tissues، cellular infilterations were observed in most of the brain areas and also binuclear hepatocytes were seen in liver tissues. These tissues characterizations were decreased in insulin and Angipars treated groups but were significantly decreased in Angipars-insulin treated group. Overall، animal tissues in insulin-Angi pars group were more similar to normal tissue. It seems that Angi pars haveing properties such as anti-oxidant and neovascularisation effects could protect the tissues from injuries in experimental group''s animals in comparison to control group animals.