The effect of human bone marrow-mesenchymal stem cells secretoms on diabetic wound healing

Diabetes is a metabolic chronic disorder that makes it difficult to heal wounds because of its complications. To this point, many studies on the use of mesenchymal stem cells in wound healing process particularly diabetic ulcers have been done. In this study, we evaluate the impact of mesenchymal stem cells-derived conditioned medium (MSC-CM) on diabetic wound strength. The type of study is experimental. 24 rats were divided into four groups: normal control, diabetic control, diabetic placebo (culture medium treated) and diabetic experimental (treated with MSC-CM). Diabetes mellitus was induced in the diabetic rat groups by intraperitoneal injections of alloxan. One full-thickness skin incision was made on the dorsal region of each rat.Conditioned medium were obtained from human bone marrow mesenchymal stem cells. 24 hours after wounding, diabetic experimental rats received 200 µl of conditioned medium, and diabetic placebo rats received 200 µl of of culture medium intravenously. 15 days after wounding, the wounds were sampled and biomechanical testing of elasticity (tensiometery) was performed on them and strength of wounds was evaluated by ANOVA test. Body weight and blood glucose difference of diabetic rats was significantly higher than the control group (P=0.00). Maximum force of experimental group was 3.45±1.05 while this criterion in diabetic control was 1.76± 0.56 and in diabetic placebo was 2.30±0.62, which was significantly increased (p<0.05). Morover,the maximum stress of experimental group was 0.34±0.1, which compared to the diabetic control group (0.18±0.05) showed a significant increase. In addition, standard elastic stiffness of the experimental group was 3.19 ±1.07, whichcompared with diabetic placebo group (2.2 ±0.19) significantly increased. It seems to be human mesenchymal stem cells-derived conditioned medium has significant effect on wound strength in diabetic rats.