Identification of compounds in the essential oil and quantification of flavonoids and Rosmarinic acid in Salvia reuterana Boiss. and Salvia palaestina Benth.

GenusSalvia، belonging to the Labiatae family، has valuable active ingredients including essential oils، flavonoids and rosmarinic acid. Pharmacological researches on Salvia genus have confirmed medicinal properties of the plant including antibacterial and antioxidant effects. Total flavonoids، rosmarinic acid and the content and chemical compounds of essential oil of three populations of Salvia reuterana Boiss.، collected from three regions of Iran (Deli chai، Kaswa and Shahmirzad)، as well as Salvia palaestina Benth.، collected from Rodbarak، were evaluated in present study. Fifty-six compounds of S. reuterana and S. palaestina were identified by GC-MS. The most important chemical compositions for S. reuterana were α-gurjunen، β-elemene، germacern-D، N-hexyl acetate and spatholenol and for S. palaestina were caryophyllen، dihydro carveol، germacern-D، linalool and spatholenol. Diethyl ether extract of leaves was used for quantification and identification of flavonoids and rosmarinic acid. The crushed leaves of the plant were dissolved in diethyl ether solvent and then were injected into the LC-DAD-ESI-MS system. Total flavonoids amount of S. reuterana، collected from Kaswa، Deli chai and Shahmirzad were calculated to be 3252. 76، 3697. 19 and 5132. 92 µg/ g، respectively. Also the amount of rosmarinic acid in plants، collected from Kaswa، Deli chai and Shahmirzad was 122، 177. 11 and 98. 46 µg/ g، respectively. S. palaestina Rodbarak showed 3808 and 19. 72 µg/g of total flavonoids and rosmarinic acid، respectively.