Effects of culture media and growth regulators on micropropagation of Gisela 6 rootstock

This study was conducted with the main prupose of investigation the effects of culture medium and the concentration of growth regulators on proliferation rate, rooting and the acclimatization of Gisela 6 rootstock at Khorasan Razavi Agriculture and Natural Resource Research Center during 2011-2012. In this experiment, the proliferation and rooting were performed in six kinds of culture medium (DKW, WPM and MS in two solid and liquid media) that supplemented with plant growth regulators included TDZ and BAP in 0, 1, 2 mg/l plus 0.01 mg/l IBA in all treatments of the proliferation and IBA with 0, 1, 2, 3 mg/l in rooting step. The results showed that the most number and length of shoot were 6.48 shoot and 3.14 cm, respectively in solid WPM medium and 2 mg/l BAP concentration and liquid MS medium with 1 mg/l concentration of TDZ. The liquid MS medium in 2 mg/l of IBA caused the highest percentage of rooting and root length, 93.90% in 11.76 cm, respectively. Although applying of the liquid media was resulted in better acclimatization of plantlets, but the highest percent of plantlets survival was obtained in WPM liquid medium with 2 mg/l concentration of IBA at about 91.80%.