Effect of Leptin During In Vitro Maturation, Fertilization and Embryonic Development of Mouse Germinal Vesicle Oocyte

The purpose of this study was to investigate the effects of leptin on in vitro maturation، fertilization and embryonic development of immature mouse oocytes. Immature oocytes were isolated from NMRI female mice and divided randomly into 4 groups: leptin 0 ng/ml (control)، 10 ng/ml، 50 ng/ml، 100 ng/ml. 24 h later matured (MII) oocytes were identified and fertilized with incubated sperm. Embryonic development was investigated every 24 h using inverted microscope. From isolated oocytes 53/1% in control group، 56/2% in first group،42/4% in second group and 38/3% in third group developed to MII. The rate of GVBD was 22/05% in control group، 31/27% in first group، 40/63% in second group and 36/39% in third group. The physiologic concentration of leptin (10 ng/ml) considerably increased the rate of MII when compared to other groups (p<0. 05)، whereas the GVBD rate was higher in group treated with concentrations more than physiologic dose. When different concentration of leptin was applied after IVF، no significant effects were observed between four treatment groups in different stages of embryonic development.