Association between XRCC1 C26304T gene Polymorphism and susceptibility to Gastric Cancer in Guilan population

Background and Aim
Gastric cancer is one of the most common malignancies and its early diagnosis can be effective in their treatment. Loss of genomic stability and the resulting gene alterations appears to be a crucial molecular and pathogenic step that occurs early in the gastric carcinogenesis process. X-ray repair cross-complementing gene 1 (XRCC1) is one of the most important DNA repair genes. The XRCC1 protein plays an essential role in base excision repair. Coding polymorphisms of the XRCC1 gene have been shown to affect the DNA repair capacity and to be associated with genetic susceptibility to carcinogenesis. The aim of this study was to investigate the association of XRCC1 C26304T gene polymorphism with the risk of gastric cancer.
In the present case-control study، genomic DNA was extracted from 110 cases with gastric cancer and 116 normal subjects as control group. Two groups had similar age، sex and ethnic background. The Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used for detection the genotype and allele frequencies of XRCC1 single nucleotide polymorphism in each subject. Data were analyzed using the MedCalc)V. 12. 1 (software and Chi-square test and P<0. 05 was considered significant.
C and T allele frequency in patients 0. 88، 0. 11 and in healthy subjects were 0. 94، 0. 06 respectively (p>0. 05). The C and T allele frequencies in cases were 0. 88 and 0. 11، respectively and 0. 94 and 0. 06 in healthy individuals (p>0. 05). The distribution of CC، CT and TT genotypes among cancer cases were 78. 2%، 20%، 1. 8%،and In the control group were 88. 8%، 10. 3%، and 0. 9% respectively. The genotype frequencies were significantly different in the cases and controls. The CT genotype was significantly associated with an increased risk of gastric cancer (p= 0. 042). In addition، the distribution of the CT+TT genotype was different between case and control subjects (p= 0. 033).
Results revealed that the Screening of XRCC1 gene polymorphism could be a marker in personal sensitivity to gastric cancer and useful in cancer treatment and prevention process. Confirmation of this finding needs to be repeated with similar studies in larger population.
Armaghane-danesh, Volume:20 Issue: 3, 2015
198 to 209  
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