ASSOCIATION STUDY OF GSTO1 GENE E155DEL POLYMORPHISM AMONG PATIENTS WITH RECURRENT MISCARRIAGE FROM NORTHWEST OF IRAN

Recurrent miscarriage (RM) is a pathologic condition that occurs in 1- 5% of couples trying to conceive and has destructive psychological effects on family life of affected couples. RM is considered as a multi-factorial disease and nearly half of RM cases cannot currently be explained clinically. Several studies have indicated that genes polymorphisms could be factors for causing susceptibility of RM with unknown etiology. GSTO1 protein has a crucial role in detoxification metabolism and it is believed that GSTO1 gene polymorphism could be involved in RM. This study aimed to investigate the possible association of GSTO1 gene polymorphism [E155del (rs 11509437)] with recurrent miscarriage from northwest of Iran was studied. In this case-control study, fifty-five patients with a history of RM and fifty-five referent women, who had at least two live births without other pregnancy complications, were included. Glutathione S- transferase Omega 1 (GSTO1) gene polymorphism (i.e. E155del) was screened using polymerase chain reaction with confronting two-pair primers (PCR-CTPP). A total of 55 patients were studied and 1.82% (1) patient for del155/del155 genotype of GSTO1 gene were homozygote (p=0.17). Allelic frequency of del155 between patients and the control groups were 9.09% (10) and 2.72% (3), respectively (p=0.023). The result of this study shows association of del155 allele of GSTO1 gene with susceptibility to develop recurrent miscarriage b ut there is no significant association between del155/del155 genotype and recurrent miscarriage in Northwest of Iran.