Frequency of Klebsiella Pneumoniae Producing Carbapenemase KPC in Clinical specimens of Mashhad during 2014

Klebsiella pneumoniae producing B-Lactamase is an important medical concern. Therefore we aimed to investigate broad spectrum lactamase KPC-producing Klebsiella pneumoniae in clinical samples in Mashhad during 2014. After identification by biochemical methods، antibiotic susceptibility of Klebsiella pneumoniae isolates against 10 different antibiotics was identified using disk diffusion technique. To evaluate the presence of carbapenemase KPC enzyme، modified Hodge test (MHT) was performed and the minimum inhibitory concentration (MIC) of Meropenem as an indicator of Carbapenem class of β-Lactam antibiotics was determined using agar dilution test. Among the isolated bacteria، high level of resistance against cefazolin (54. 44%)، cefepime (49. 46%)، ceftazidime (45. 19%)، trimethoprim-sulfamethoxazole (46. 26%) was observed. Modified Hodge test was performed for all 136 ESBL Klebsiella pneumoniaisolates and 20 were found to be positive for MHT. Furthered MIC test revealed that 2 isolate had MIC=4 μg/ml، 3 isolate had MIC=2، and remaining 15 isolates had MIC more than 8. This study underscores the risk of increasing carbapenem resistant Klebsiella pneumonia specieswhich demands further attention in clinical settings.