Assignment of AFLP Markers to Linkage Groups in Doubled Haploid Lines of Wheat

Molecular mapping and construction of linkage maps in plants genome play important role in marker assisted selection and breeding programs of target traits. Eleven combinations of PstI and MseI primers were used to assign AFLP markers to the wheat (Triticum aestivum L.) linkage groups in 107 doubled haploid (DH) lines. The results of AFLP analysis indicated that amplified fragments (66 polymorphic bands) length of the primer combinations varied between 100 and 1000 base pairs (bp). Out of the 66 AFLP markers, 9.0% showed segregation distortion in the DH population that discarded from the linkage analysis. Linkage analysis for AFLP markers showed that after eliminating deviated markers from 1:1 segregation ratio, number of assigned markers to B and A genomes were respectively 21 (43%) and 20 (41 %), and a number of 6 markers were assigned to D (12%) linkage groups. A number of 12 markers were not assigned to any of wheat linkage groups. Number of assigned markers to the wheat homeologous chromosomes of 1 to 7 were 6 (12.5%), 7 (14.5%), 4 (8.7%), 5 (10.4%), 9 (18.7%) and 12 (25%), respectively. The highest number of AFLP markers were assigned to chromosomes 7A (7 markers) and 1B (5 markers). Among the mapped AFLP markers, P06m19-7-4, P01m19-8-6, P04m18-9-5, P03m22-9-6, P08m22-11-4, P03m22-8-3, P04m22-9-4, P01m24-8-2, P01m24-8-4 were located on those regions of A, B and D linkage groups that saturated the low dense intervals in previously available map and therefore, showed a better coverage on wheat genome compared with other AFLP markers.