Isolation and culture of mouse newborn skin fibroblast cells in a simple and inexpensive method
Abstract:
Background and
Objective
Cell culture is one of the main techniques used in scientific researches that done in three ways: Primary culture, secondary culture or cell passage and purchase Cell Lines of Cell banks. Fibroblast cell is an important component of connective tissue that is found almost everywhere in the body. Fibroblast cells based on their location have functional and morphology roles. The aim of this project was simplification through innovation and localization, and to obtain mouse newborn skin fibroblast cells in order to use these cells for further study.
Methods
For this project were used from mouse neonatal that is one to two days. Initially all devices, including surgical materials, culture dishes, buffer and etc were sterilized by autoclaving at 121 ° C and intense pressure. As well as workplace were sterilized by 70% alcohol and UV. Initially mouse neonatal under the biology hood and in 70% alcohol were killed and were sterilized with sterile instruments and then skin fibroblast cells were isolated from these mice. In addition skin fibroblast cells for further growth were transferred of the primary environment to a fresh and new medium and first passages was performed on these cells.
Results
Fibroblast cells in different days were evaluated in terms of growth and morphology by fluorescent microscope (Olympus) with a magnification of 10, that this cells in terms of morphology were appropriate.
Conclusion
According to the results of this study, the simplicity of this method, no need to high costs and sterile conditions without the use of antibiotics and anti-fungal, makes reasonable use of this method for isolation and cell culture in different studies.
Language:
Persian
Published:
Pars Journal of Medical Sciences, Volume:14 Issue: 3, 2016
Pages:
35 - 42
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