Evaluation of total phenol and flavonoid content, antioxidant activity, anti-pain and anti-inflammatory of Ocimum basillicum var. purpurescens in comparison with opioid drug (morphine) in male rats

This study was carried out to evaluate the total flavonoide, phenols, antioxidant, anti-pain and anti-inflammatory in different extracts of Ocimum ebasillicum var. purpurescens in comparison with opioid drug (morphine) in male rats. The male Wistar rats with 250±10 g weighing was used and randomly divided to five groups. Group I (treated by 0.1mg/kg acetic acid, II-IV (treated by 0.7, 0.9 and 1 mg/kgof plantextract) , V (treated by 0.1 mg/kg morphine) and evaluated by Writhing test. The total phenol and flavonoids content was evaluated by follin-ciocalteus reagent, Ammonium chloride, the antioxidant activity was measured by DPPH method and serum levels of PGE2 and SOD activity measured by ELISA Kits. According to results, the ethanol extract of plant had more content of secondary metabolites with total phenol (288.3±51.5 mg EGA/g) content, flavonoide content (154.7±1.04mg EGU/g) and the highest ability of free radicals scavenging (79.4±4.1%). There was a significant correlation between phenol- flavonoid ingredients to antioxidant activity rate and free radicals scavenging of plant (P