Isolation and Identification of Mycobacterium tuberculosis complex bacteria from rodents of infected cattle farms in Khuzestan Province

The Mycobacteria grouped in the Mycobacterium tuberculosis complex are causes of Tuberculosis in animals and humans. This chronic disease also affects a wide range of other domestic and wildlife animals and may also cause disease in humans. It is very important to control and prevent the epidemic among human and animals because of zoonotic identity of the pathogen and several cattle flocks in suburb of cities, and it must be done better through more active surveillances. During this study, the causative agent of disease must be isolated from rodents found and hunted on the reactor or suspected farms. Finally, it must be distinguished that which isolates (species) are circulated in those areas. This study has been carried out by cooperation of Khuzestan Veterinary Office. By use of T.B test sample Farms have been selected. They consist of 16 mice from the same farms. All samples were referred to laboratory, then cultured in specific Lowenstein-Jensen slant media. After at least 8 weeks incubation (at 37ºc) DNA was extracted from 2 isolates of 16 mice to identify the isolates belonging to the Mycobacterium tuberculosis complex, by using PCR detection of insertion sequence 6110 (IS6110) and (16srRNA). According to the test 2 isolates of mice belong to the family of Mycobacterium tuberculosis complex.