Effect of intramuscular and intravenous injection of rosemary (Rosmarinus officinalis L.) extract on shelf life of rainbow trout (Oncorhynchus mykiss) fillet in refrigerated storag

This research was performed to investigate the effect of rosemary (Rosmarinus officinalis L.) water extract on shelf life of rainbow trout fillet in the refrigerator (4±1ºC) based on total microbial load, chemical evaluation and sensory characteristics. For this reason, the fish were divided into three groups including fish with intravenous injection of rosemary extract at the rate of 0.1% and 0.3% (treatment 2 and 3), intramuscular injection of rosemary extract at the rate of 0.1% and 0.3% (treatments 4 and 5) and non-injected (treatment 1). Fish were caught and filleted 62 hours after injection, then kept for 16 days at refrigerator. During this period, peroxide value (PV), thiobarbituric acid (TBA), free fatty acids (FFA), total volatile nitrogen base (TVB-N) and total bacterial load (TVC) indices were measured every 4 days and also sensory evaluation was performed. According to the results, treatments 3 and 5 showed significant differences (P