The effect of concurrent aerobic training with Origanum vulgare Hydro Ethanolic Extract supplementation on gene expression of myocardial apoptosis proteins in male rats

Today, the use of supplements and herbal extracts has been widely used to prevent exercise induced apoptosis and improve exercise training adaptations. Thus, the present study examined the
effect of the 12 weeks aerobic training with Origanum vulgare Ethanolic Extract supplementation on the myocardial apoptosis in young male rats. In this experimental study, 40 male Wistar rats (8 weeks old) with an average weight 129±17.86 gr randomly divided in five equal groups: basic (n=8), control (n=8), Origanum vulgare (n=8),
aerobic training (n=8) and aerobic training- Origanum vulgare (n=8). The aerobic training and aerobic training- Origanum vulgare groups participated in a 12-week program (5 sessions of 10-60 min each week, with an intensity of 75-80% of maximum oxygen consumption) running on animal treadmill (15% incline and 24-33 m/s). Also, Origanum vulgare Ethanolic extract was used for supplementation. Forty-eight hours after the last exercise session, a part of the left venticular tissue of the heart was removed and expression of Bax, Bcl2 and caspase-9 proteins was investigated using RT-PCR method. Data were analyzed by Two-way ANOVA test (P<0.05). The results showed that expression of Bax, caspase-9 and Bax/ Bcl-2 ratio in the training and training- Origanum vulgare groups was lower than the control group and expression of Bcl-2
protein was higher than the control group, but this difference was not significant. In addition, the difference between these groups in all Based on these findings, it seems that 12 weeks of aerobic training with supplementation of Origanum vulgare extract did not lead to apoptotic processes and improved mitochondrial
adaptations. Therefore, more research is needed to achieve better and clearer results.