LncRNA TERRA expression level and telomere length in placenta samples of healthy and preeclampsia women
Message:
Abstract:
Introduction

Preeclampsia is a specific pregnancy syndrome that affects all parts of the body. One of the possible causes of preeclampsia is genetic factors, including genetic predisposing genes. The aim of this study was to compare the expression level of LncRNA  (large non coding RNA) TERRA (Telomeric repeat-containing RNA) and telomere length in a pair of women with preeclampsia and healthy.

Methods

In this case-control study which was performed in 2017-2018, placenta specimens were taken from 25 mothers with Pre-eclampsia who referred to Ardabil Alavi Hospital and were candidate for pregnancy termination; in the control group also, placenta specimens were taken from 25 women without preeclampsia. After extraction of RNA and cDNA synthesis, TERRA expression level was measured by Real Time PCR and the changes of Telomere length was measured by Telomere Length Assay technique. Data were analyzed by SPSS software (version 16) and Independent t-test. P

Results

The mean for expression level of LNCRNA TERRA in the case group was 7.87 ± 1.9 and in the control group was 9.8 ± 2.8. Independent T-test results showed significant difference between the two groups in terms of expression level of LNCRNA TERRA (p=0.006). The mean for Telomere length in the case group was 4.35 ± 1.9 and in the control group was 6.2 ± 1.54. Independent T-test results showed significant difference between the two groups in terms of Telomere length, too (p=0.001). There was no significant correlation between maternal age and LNCRNA TERRA expression level and telomere length (p = 0.88). There was no statistically significant difference in expression level of LNCRNA TERRA and Telomere length between the sexes of newborns (p=0.1).

Conclusion

Our data identified the significant role of LNCRNA TERRA and Telomere length in Pre-eclampsia. LNCRNA TERRA and Telomere length can be used to predict and diagnose preeclampsia

Article Type:
Research/Original Article
Language:
Persian
Published:
Iranina Journal of Obstetrics Gynecology and Infertility, Volume:23 Issue: 3, 2020
Pages:
9 - 18
magiran.com/p2143663  
برخی از خدمات از جمله دانلود متن مقالات تنها به مشترکان مگیران ارایه می‌گردد. شما می‌توانید به یکی از روش‌های زیر مشترک شوید:
اشتراک شخصی
در سایت عضو شوید و هزینه اشتراک یک‌ساله سایت به مبلغ 400,000ريال را پرداخت کنید. همزمان با برقراری دوره اشتراک بسته دانلود 100 مطلب نیز برای شما فعال خواهد شد!
پرداخت با کارتهای اعتباری بین المللی از طریق PayPal امکانپذیر است.
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی همه کاربران به متن مطالب خریداری نمایند!
توجه!
  • دسترسی به متن مقالات این پایگاه در قالب ارایه خدمات کتابخانه دیجیتال و با دریافت حق عضویت صورت می‌گیرد و مگیران بهایی برای هر مقاله تعیین نکرده و وجهی بابت آن دریافت نمی‌کند.
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.