Evaluation of inactivated Vibrio fluvialis vaccine in Hyline W36 breeding hens

Vibrio fluvialis causes disease and significant economic losses in breeding fish. This bacterium is considered as a human pathogen and several species of marine spawning fish. The egg yolk immunoglobulin technology (IgY) has been used to control many microbial infections. The purpose of the present study was to evaluate the vaccine prepared from V. fluivialis bacteria after injection into laying hens. V. fluvialis was admixed by 0.05% formaldehyde after culture in a liquid medium. Then, the pathogen along with Freund’s adjuvant was injected to the Hyline W36 hens as a vaccine for 7 days. Increase of the weight of the hens was measured until Physical maturity and egg production. Then, an Indirect ELISA was designed to detect IgG Anti V. fluivialis. Injection of the vaccine had no significant effect on weight gain of hens and the number and weight of eggs during the experiment. ELISA test showed that antibody titers in the serum of hyper-immunized hens after injection of the fourth booster reached 1: 12800. According to the results obtained and the results of studies by other researchers, egg yolk antibody (IgY), which is the same as IgG, has been shown to inhibit the pathogenicity in lab and farm animals.