The Effect of aerobic training and octopamine on HSP70 and Caspase-3 protein expression in brown adipose tissue in rats received deeply heated oil treatment

Deep heated oils produce toxins that endanger peoplechr('39')s health. The use of herbal supplements along with exercise can improve general health. Therefore, the present study aimed to investigate the effect of aerobic and octapamine training on HSP70 and Caspase 3 protein in brown adipose tissue of rats received w deep heated oils.

40 male wistar rats after four weeks of feeding with heated oil were randomly assigned into control-intoxication (CI; n=8), exercise training-intoxication (ETI; n=8), supplement-intoxication (SI; n=8), supplement-exercise-intoxication (SEI; n=8), and healthy-control (HC; n=8).  The exercise-training was done for four weeks at intensity of 50-65% vo2max for 20 minutes per session. Octopamine was used as a supplement for 4 weeks and it was also used in the form of intraperitoneal injection with a dose of 81 µmol/kg for five days per week in the CI, SI, and SEI. To test the HSP70 protein by immunohistochemistry And Caspaz 3 used Western blot.

The results showed that aerobic exercise significantly reduces HSP-70 protein (P = 0.001). Octopamine intake significantly reduced HSP-70 protein (P = 0.001). However, the interaction between aerobic exercise and octopamine did not have a significant effect on HSP-70 protein concentration. Aerobic exercise significantly reduced Caspase 3 (P = 0.001). Taking octapamine supplementation significantly reduced caspase 3 (P = 0.001). Also, the interaction of aerobic exercise and octopamine significantly reduced Caspase 3 (P = 0.016).

Aerobic exercise and octopamine reduce the detrimental effects of deeply heated oils. It is recommended to use food at a temperature below 180 0C and in order to reduce the effects of toxins produced by these oils, regular aerobic exercise and octapamine supplementation would be useful.