The effect of catechin metabolism derived from intestinal microbiota on Ca SKi cell proliferation

In Iran, cervical cancer is the second leading cause of death in women. Papillomavirus infection is the most important risk factor for the development of cervical cancer. Ca SKi cell is a human cervical carcinoma cell that binds the papillomavirus type 16 virus to its genome. Cactin is a type of phenol and a secondary metabolite of a plant. The purpose of this study was to investigate the effect of cutaneous metabolites from the gut microbiota on the proliferative power of the Ca SKi cell line.

Catechin metabolites were extracted from fecal samples containing intestinal microbiota by HPLC method and were treated with different concentrations of catechin for 24 and 48 hours. The cytotoxic effects of the extract of Ca SKi bacterial cell line were evaluated by MTT assay. After RNA extraction and cDNA preparation, theexpression of bax, bcl2 and bax2 gene in Ca SKi cell line were evaluated by Real Time PCR..

The expression of bax gene did not show a significant correlation with the effective dose of catechin in the first 72 hours (p> 0.05). However, with increasing dosing, the effect of the control showed a significant increase in the expression of the bax gene. The results showed that expression of bcl2 gene with a significant increase in the effective dose of catechin was significant in the first 72 hours. This decrease is significant and has a direct correlation with dosage.

Catechins can enhance the expression of bcl2 in cancer cells, which can be used to create a high-impact new therapies as a cure for prevention of cervical cancer and bax gene as a prognosis for cervical cancer.