Study of the expression level of microRNA-4443 in tissue samples paraffinembedded ovarian cancer comparison with non-tumor samples

Ovarian cancer (OC) is one of the most lethal malignancies in women. Hence, new investigative and therapeutic methods for OC are instantly needed. The use of new biomarkers for faster diagnosis and selection of more efficient therapies is one of the main concerns in this area. Few studies have been conducted on the effects of miR-4443 in cancer. Therefore, to determine the role of thismiRNA in OC, this study was directed to investigate the expression rate in OC tissue samples and its relationship with clinical factors.

In this study that was descriptive-analytic, regarding the role of miR-4443, its expression levels were evaluated in 35 ovarian tumor and 35 ovarian non-tumor tissue samples paraffinembedded by using qPCR. Expression was investigated by miRNA specific primers and then statistical analysis was performed to determine the significance. In the next step, the relationship between clinopathologic factors and miRNA expression was investigated.

The results of the study showed that miR-4443 decreased in OC and in metastatic samples (P<0.0001).

As a result, this studied miRNA may contribute to suppressing tumor. Therefore decrease in its expression is associated with increased cell proliferation and invasion. Further investigation can help to suggest thismiRNA as diagnostic biomarker or therapeutic target in OC.