Cytotoxicity effects of Extract and Biomass of the Wild and Mutant Types of Bradyrhizobium japonicum on the Breast & Stomach Cancer Cells

Stomach cancer and breast cancer are most common cancers in the world and in Iran. Today, the use of fungal and bacterial extractions has been interesting to cancer researchers. Rhizobium is a highly beneficial bacteria in agriculture and environment that provides the nitrogen needed for Legume family plants through coexistence with this family.

In this study, the effect of Bradyrhizobium cell extraction and its mutated type on the cancer of breast (MCF7) and stomach (AGS) was investigated.

Cytotoxicity effect of different concentration of Bradyrhizobium japonicum biomass and extract on stomach and breast cancer cells was investigated using MTT colorimetric method. Data analysis were conducted using Graphpad Prism 6.0 software and ANOVA method.

The findings showed that both wild type and mutant type of bacteria had a significant meaningful effect on both types of cancer cells and their cytotoxicity was dependent on the concentration of extract or biomass (the lowest rate was observed at a concentration of 30 and at most 480 Micrograms per ml). In most cases biomass showed a better effect than  extract.

In general, this study showed the effect of extract and biomass of Bradyrhizobium bacteria cytotoxicity on cancer cells, which could be used as a source for antineoplastic agent.