IN VITRO EVALUATION OF ANTIOXIDANT CAPACITY OF THYMUS KOTSCHYANUS HYDRO-ALCOHOLIC EXTRACTS AND ITS EFFECT ON SERUM PARAOXONASE 1 ACTIVITY IN DIABETIC AND HEALTHY PERSONS

Thymus species have significant amounts of phenolic and flavonoid compounds and demonstrate strong antioxidant activities. Paraoxonase1 act as antioxidant enzyme and protect the low-density lipoprotein against oxidation. In our study we aimed to evaluate the antioxidant capacity of Thymus Kotschyanus Hydroalcoholic extract and its effect on serum paraoxonase 1 activity in healthy and diabetic person.

The antioxidant activity, and functional groups of the constituents in T. Kotschyanus Hydroalcoholic extract were determined using DPPH free radical scavenging assay, and The FTIR spectroscopy, respectively. Paraoxonase-1 activity was determined in 40 healthy and diabetic persons by measuring the rate of paraoxon hydrolysis substrate to p-nitrophenol, which absorbance was monitored at 405 nm. The data Statistically were analyzed by Duncanchr('39')s and independent t-test.

The IC50 values (the concentration with scavenging activity of 50%) was found to be 477.5 μg/ml. FTIR spectrum analysis showed biomolecules containing a hydroxyl group and aromatic ring in T. Kotschyanus hydroalcoholic extract. Serum paraoxonase activity in healthy and diabetic humans exposed to the extract at concentration of 1 mg/mL increased by 49.95 ± 3.57% and 51.05 ± 3.25%, respectively. Although there was a significant difference between serum enzyme activity in healthy and diabetic subjects in the presence and absence of the extract but the amount of enzyme activation affected by the extract in two healthy and patient did not show significant difference.

This plant extract increased enzyme activity due to the antioxidant properties and the presence of phenolic compounds in the plant extract.