Effects of seed priming with some plant growth regulators and fertilization with organic manure on the chemical composition, in vitro gas production parameters and digestibility of lentils

A study was conducted to evaluate the effect priming and inoculation with bio-fertilizers on the chemical composition and in vitro fermentation parameters of lentil. The lentil seeds to priming treatments, with two percent sodium hypochlorite disinfection solution, and cultured for 8 hours before the desired solutions (hormone gibberellic acid, and salicylic acid and distilled water) were kept. Treatments were: The first factor is the use of mycorrhizae in three levels [Non-inoculated (control), inoculation with mycorrhizal fungi species Glomus intraradices and G. mosseae]. The second factor involves priming treatments in 5 levels [Hydro-priming (using water), priming with gibberellic acid 100 ppm, Priming with salicylic acid 100 ppm, Priming with gibberellic acid 100 ppm × priming with salicylic acid 100 ppm and control (no treatment)]. The chemical composition of the samples was determined using the standard methods. In vitro digestibility of samples was determined by the batch culture method. Results showed that there was significant difference among treatments on chemical composition. In the absence of fungus, treatment combination of two hormones had most and salicylic acid and gibberellic acid had lowest crude protein content. In this study, the lowest neutral detergent fiber and acid detergent fiber content was related to treatment Glomus intraradices Glomus intraradices × salicylic acid. Results showed that pre-treatment of lentil seed with salicylic acid and combination of two hormones in drought conditions, compared with Glomus intraradices and Glomus mosseae, increased crude protein, neutral detergent fiber and nutrients digestibility. The highest apgas production potential (294.9 ml) and metabolizable energy (13.48 Mj/kg) were related to treatments Glomus mosseae and Glomus mosseae × gibberellic acid, respectively. Totally, the obtained results of this study showed that any treatment of crop seeds for resistance to diseases, pests and stresses such as salinity and drought, making ability of roots in the absorption of water and nutrients can affect nutritive values of their forages and other by- products.