Optimizing of Eruca sativa (khandal) Extraction by Microwave Method and its Application in Marinated Huso huso (Beluga) Fillet

Marination accompanied with the application of functional plant extracts is aconvenient method to preserve fish meat. The present study aims to extract Khandalcontaining antioxidant compounds by the use of microwave and applying it to marinatedfillets of Beluga in order to increase its shelflife at 4±1ºC. Microwave extraction optimization according to the solvent type,solvent ratio (5–15.5cc), time (10-30min) and power (90-270W) through RSM and applyingthe optimum extract in respect to the total phenolic compounds and the inhibitory potential ofDPPH free radicals for preparing marinates (i.e. control, treatments containing10 (T1),20 (T2),and 30% (T3) extract( and storage during 20days at 4±1ºC and monitoring the physical,chemical and sensory properties and comparing the results and data were carried out usingone-wayANOVA and chi-square methods. The results indicated that the optimal extract was obtained by the use of water for30min, at 90W and solvent ratio of 5. Moreover, it was revealed that TBA inT2wassignificantly controlled as compared to other groups and the amount of TVB-N, in T2 and T3were lower significantly (P<0.05) and being controlled. In terms of water activity, the highestamount was observed in T2 at days 0 and 10. The highest WHC was observed in the controlon day 0, which was decreased during storage time. The texture hardness, color, appearanceand odor didn’t show significant difference among the groups, but taste and oral sensationwere differed significantly over time. In terms of texture analysis, due to excessivemarinating, the samples became soft and watery and low score was obtained. Examination ofacid-salt taste showed that the marinated samples had acidic and salty taste. It is concluded that the sample containing 20% of extract was selected as thebest group in controlling chemical spoilage.