Antioxidant Effects of ‎C-Phycocyanin in Colon Cancer ‎Cell Lines invitro and invivo

C-Phycocyanin has been demonstrated to have a series of pharmacological attributes without leading to toxicity. The aim of this study was to evaluate potential of anti-cancer and antioxidant C-PC on CT-26 and HT-29 cell lines in vitro and in Balb/c mice. The CT-26 and HT-29 cells were treated with various concentrations of C-PC extract (1-100µg/ml) in 48hr. Antiproliferative effect was measured by morphological observations, DAPI and AO/PI Staining, MTT assay, fluorescence microscope and Flow cytometry assays. Antioxidant effects of C-PC on CT-26 tumor cells transplanted in Balb/c mice was also checked out invivo. Male Balb/c mice were tested in four groups.group1 was considered as control. Group 2 were fed by C-Phycocyanin (50mg/kg) daily. In groups 2-4, cisplatin (3mg/kg) was injected, and group 3 silymarin (100mg/kg) was injected daily. Finally serum levels of MDA, TAC, and Total Billirubin, Total Protein and Albumin and activities of GPX, Catalase, SOD, ALT, AST, and LDH were assayed. C-PC showed considerable anti-proliferative effect on CT-26 and HT-29 tumor cell lines with IC50 =47.4 µg/ml and IC50=49.4 µg/ml respectively. In addition, C-PC because of its antioxidant potential, significantly (P<0/001), decreased MDA and increased levels of liver antioxidant enzymes.