Improve the composition of Daphnia (Daphnia longspina) Fatty Acids using bioenrichment

The purpose of this study was to isolate and cultivate of microalgae enrich of fatty acids in order to improve the composition of fatty acids in daphnia. Scenedesmus dimorphus and Chlorella vulgaris were isolated and cultivated.  For this study two treatment groups and one control group (with three replicates) were considered. Daphnia in treatments 1 and 2 were fed with S. dimorphus and C. vulgaris, respectively and control group didn’t feed any algae. The microalgae density to enrich of daphnia was 5×107 cells/ml. Daphnia enrichment was carried out for 24 hours. On the basis of results, the amounts of PUFA in S. dimorphus and C. vulgaris were %16 and %26.3 respectively. The amounts of unsaturated fatty acids of Eicosapentaeonic acid (C20:5) and Docosahexaenoic acid (C22:6) in S. dimorphus, were %0.8 and %1.1 respectively. The amounts of the Linoleic acid (C18: 2) and Linolenic acid (C18:3) in C. vulgaris were %8.6 and %16.9 respectively. The PUFA amounts in control group, daphnia enriched with C. vulgaris and daphnia enriched with S. dimorphus were %15.1, %17.6 and %19.8 respectively. Enrichment of Daphnia with the mentioned algae was effective in improving fatty acids composition of daphnia.