Effect of Aerobic Training and High-fat Diet on Enos and Ros in Testicular Tissue of Juvenile Male Rats

This study aimed to determine the effect of a course of aerobic exercise with a high-fat diet on eNOS and ROS in testicular tissue of adolescent male rats. 

A total of 40 adolescent male rats (30 days old) were randomized in the following groups: normal diet control, normal diet training, high fat diet control, and high-fat diet training. The high-fat diet rats were under a high-fat regimen (5.817 kcal/g) for 30 days, and then a normal fat diet (3.801 kcal/g) was continued after the 60th day of birth. Aerobic training was conducted for four weeks included three training sessions from the 70th to 98th days of life. 

The results showed that the amount of ROS in the testicular tissue of male mice was higher only in the high-fat diet group. Also, there was no significant difference between the groups regarding eNOS testicular tissue in male mice.

 A high-fat diet increases the production of reactive oxygen species in testicular tissue and is not affected by aerobic exercise. Also, neither exercise nor a high-fat diet had any effect on testicular eNOS. However, due to the limitations of this study and no evidence in this field, further studies are needed on cell phenotype, sperm fate, and identification of pathways involved in the occurrence of oxidative stress and subsequent effects of eNOS activation in testicular tissue in response to exercise and obesity.