The effect of eight weeks of continuous and interval training with warfarin on expression of cardiac osteopontin gene and coagulation indices in male Sprague-Dawley rats with myocardial infarction

Myocardial infarction, in addition to destroying cardiac tissue remodeling, also affects the blood homeostasis system. The purpose of the present study is to investigate the effect of eight weeks of continuous and interval training with warfarin on expression of cardiac osteopontin gene and coagulation indices (PT, PTT, INR) in male rats with myocardial infarction.

In this experimental study, 42 male rats (eight weeks old) were randomly divided to seven groups of (six in each) healthy control, myocardial infarction, myocardial infarction with (interval training, ‌continuous training, ‌warfarin, ‌interval training+‌warfarin, and ‌continuous training+‌warfarin). Duration of incremental interval training (one minute: 16-33, two minute: 10-14) m/min and incremental continues training (14-24 m/min, 10-60 minutes) was eight weeks, five sessions per week. Induction was induced by subcutaneous injection of isoproterenol in two doses of 85 mg/‌kg. 48 hours after the last training session, blood samples and heart tissue were extracted to measure the variables. Independent T-test, one-way ANOVA and Two-way analysis of variance were used to analyze the data (P<0.05).

Induction of myocardial infarction leads to increased expression of osteopontin (P=0.001), PT values (P=0.001), PTT (P=0.001) and INR (P=0.001) compared to the healthy control group. The results of Tukey test showed that PT values in myocardial infarction+warfarin group (P=0.002) and myocardial infarction+warfarin+ECT group (P=0.001) had a significant decrease compared to myocardial infarction group. There was a significant decrease in PTT values in of all groups (P<0.05) and also a significant increase in INR of the experimental groups that had received warfarin compared to the myocardial infarction group (P<0.05).

In myocardial infarction, interval and continuous training along with warfarin can control PT and PTT coagulation factors. Further studies on the replacement of osteopontin with therapeutic modalities of the present study are needed.