The effect of aerobic training and stevia on the expression of miR-322 and cyclin D1 in heart tissue of type 1 diabetic rats

A close link exists between diabetes and cardiovascular disease. cardiovascular disease is the most prevalent cause of mortality and morbidity in diabetic populations. The response of biomarkers such as miRNA to environmental factors is important for optimizing diabetes treatment strategies. This study aimed to evaluate the effect of aerobic training and stevia supplement on the expression of miR-322 and cyclin D1 in heart tissue of type 1 diabetic rats.

In this experimental study, 25 type 1 diabetic rats (weight 300-250 g) were divided into 5 groups; healthy control, type 1 diabetic control, type 1 diabetic – stevia supplement, type 1 diabetic-exercise and type 1 diabetic - exercise - stevia supplement. Aerobic training was performed 5 days a week at a speed of 20 to 30 meters per minute and a slope of zero degrees for 8 weeks. The dose of stevia was 250 mg per kilogram of body weight in gavage. Rats were slaughtered 48 hours after the last training session and Cardiac tissue was used to analyze the indicators. The gene expression of miR-322 and cyclin D1 in cardiac tissue was measured by Real Time PCR. Data were analyzed by One-way ANOVA and Bonferron's post hoc test at the P<0.05.

Aerobic training, stevia and the interaction of aerobic training and stevia supplementation had no significant effect on the gene expression of miR-322 and cyclin D1 in the heart tissue of type-1 diabetic rats (P<0.05).

According to the results of the study, it seems that intervention of aerobic training with stevia supplementation has no effect on the gene expression of cardiac metabolic markers in type 1 diabetes.