Design of immunosuppressive structure based on Spike protein (s) virus Corona covid 19

The human coronavirus is a member of the Coronaviridae family and causes upper respiratory tract infections. Despite repeated severe epidemics and the lack of appropriate antiviral drugs, not much progress has been made on the epitope-based vaccine designed for HCoV.

: The method of this study was to select the spike corona virus protein sequence from NCBI, retrieve the protein sequence and determine the T, B epitopes required to produce the chimer vaccine, evaluate the antigenicity and allergenicity and toxicity of the selected epitopes, respectively. Different servers were designed to configure the primary chimer composition of the epitope vaccine. Then, the chimer vaccine was evaluated in terms of structure and connectivity to B cells and MHCI and II compounds, and the two-dimensional structure and position of amino acids and bonds in the immunogenic model were studied, as well as the physicochemical and stability of the model vaccine by some other servers. Finally, it was tested for binding against HLA molecules using silico docking techniques to investigate the interaction with the epitope.

The results showed that the immunogenic construct created in terms of two-dimensional and three-dimensional structure and the position of amino acids and bonds in the model of immunogenic structure, toxicity and allergenicity and antigenicity were in good condition. And had stability (instability index 33.93) and favorable half-life and suitable physicochemical conditions.

In general, the immunogenic structure that was prepared in this research process could have a favorable interaction with some components of the immune system (HLA) in the docking process, which indicates the optimal identification of this structure by the humoral and cellular immune system and stimulation in In order to produce immunity in the body of the host, of course, more reliable proof of it requires clinical phase processes.