The Effect of Eight Weeks Endurance Training, Somatropin Injection, and Its Lipolytic Fragment (AOD9604) on Cytokeratin-18 and Liver Enzymes of Mice Induced Liver Damage Due to a High-Fat Diet

Fatty liver and its treatment are among the concerns of today’s society. So this study aimed to investigate the effect of endurance training, Somatropin injection, and its lipolytic fragment (AOD9604) on cytokeratin-18 (CK18) levels and liver enzymes of mice with fatty liver.

 In this experimental study, 28 male mice were randomly divided into four groups (7 mice in every group): control (C), exercise (E), exercise + fragment (EA), and exercise + growth hormone (EGH). A medium-intensity endurance training program was performed for 8 weeks, 5 sessions per week with an intensity of 50% VO2max. The somatropin and fragment injection protocols were 1 mg and 250 mc/kg of body weight, respectively. The mice were evaluated 48 hours after the last training session. The obtained data were analyzed using 1-way ANOVA and post hoc Tukey tests at the significant level of P<0.05 in SPSS version 26.

CK18 showed a significant decrease in group E compared to the control group only (P=0.00). CK18 values were significantly higher in the EGH group compared to the E (P=0.00) and EA (P=0.04) groups. HOMA-IR (homeostatic model assessment for insulin resistance) index had a significant decrease in the E (P=0.00) and EA (P=0.03) groups, but no significant changes were seen in the EGH group. Changes in aspartate transaminase were not significant in any of the groups. Alanine transaminase levels in all three groups of E (P=0.00), EGH (P=0.03), and EA (P=0.00) were significantly lower than those in the control group, but the inter-group changes were not significant.

 Endurance training has produced a more effective response in improving nonalcoholic fatty liver disease markers than GH and fragment peptides. Growth hormone injection can have negative consequences on some indicators of this abnormality.