Study of factors affecting on the micropropagation of the MA×MA60 sweet cherry ‎semi-dwarf rootstock ‎

MA×MA60 is a sweet cherry semi dwarf rootstock that is easily reproduced through micropropagation and because of the resistance to phytophytora, which is one of the most important factors in the deterioration of stone fruit trees, it is a valuable rootstock. The aim of this study was to investigate the effect of culture media and plant growth regulators on the proliferation and rooting of MA×MA60 rootstock at the Khorasan Razavi Agricultural and Natural Resources Research and Education Center. This experiment was conducted as a factorial in a completely randomized design with 4 replications. At the proliferation stage, the treatments consisted of three culture media (MS, WPM and DKW) and two growth regulators (benzyl amino purine and thiadiazuron) at concentrations of 0, 1 and 2 mgL-1 and at the rooting stage, treatments consisted of three culture media (MS, WPM and DKW) and different concentrations of indole-butyric acid (0, 1, 2 and 3 mgL-1). The results showed that in the proliferation stage, the highest number of shoots (33.6), leaves (20.33) and plantlet quality (3) were recorded in MS medium enriched with 2 mgL-1 of benzyl amino purine, and the shoot length (3.2 cm) in the MS medium enriched with 1 mgL-1 thiadiazuron. The highest rooting percentage (75%) and root number (5.33) were obtained in MS medium enriched with 3 mgL-1 indole buteric acid and DKW enriched with 2 mgL-1 indole buteric acid, respectively. Plantlets successfully adapted to the greenhouse conditions. Survival rate was about 75% in cocopeat and perlite culture.