Optimization of Ultrasound-assisted Extraction of Fenugreek Seed Protein and Evaluation of Its Structural, Functional Properties and Antioxidant Activity

Article Type:
Research/Original Article (دارای رتبه معتبر)

Proteins are one of the most important food constituents due to their unique functional properties and health benefits. Among plant proteins, fenugreek seed protein is of great interest due to its high content of essential amino acids. The current study was carried out to optimize ultrasound-assisted extraction of fenugreek seed protein and evaluation of its functional, structural and antioxidant properties. The optimal conditions for protein extraction were ultrasonic power of 80 W and sonication time of 28.39 min which under these conditions, the predicted extraction yield was 8.65% which was in good agreement with the experimental value (8.57%). Amide A, amides type I, II, and III bands were observed in Fourier transform infrared spectrum. The solubility was pH-dependent. The water and oil absorption capacity was 6.24 and 2.72 mL/g, respectively. The lowest (44.66%) and highest (92.49%) foaming capacities were observed at pH 4.5 and 10, respectively. The highest emulsifying capacity (84.53%) was observed at pH 10, while the lowest (54.82%) was at isoelectric point. At the constant pH, the foam and emulsion stability decreased over time. The results revealed that ultrasound can be applied effectively to reduce the time of protein extraction process from fenugreek seeds. In addition, the fenugreek seeds extracted by using ultrasound have desirable functional and antioxidant properties that make it feasible for utilization in the food industry.

Food Science and Technology, Volume:19 Issue: 5, 2022
39 to 55
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یک‌ساله به مبلغ 1,390,000ريال می‌توانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
  • حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران می‌شود.
  • پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانه‌های چاپی و دیجیتال را به کاربر نمی‌دهد.
In order to view content subscription is required

Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!