Comparison of Thymus vulga and Rosmarius officinalis extracts with salicylic acid, on mechanical transmission of Cucumber Mosaic Virus

The Cucumber Mosaic Virus (CMV) belongs to the Cucumovirus that is one of the most important viruses that infect cucurbit plants in Iran and around the world. This study was accomplished in a completely randomized design by 6 repeats to survey spraying herbal extracts, salicylic acid, and distilled water as the control on resisting cucumber plants against CMV mechanical infection in a greenhouse at the Islamic Azad University of Jahrom Branch in 2015. It was planted 4 seeds of cucumber in each pot, and at the time of inoculation 2 plants were reminded. After treatments, (including spraying of 3% thyme and Rosemary extracts, 0.02% salicylic acid, and distilled water) the plants were inoculated mechanically by the sap of an infected plant. After that, pots were checked for a month. The infection of plants examined by the ELISA test. Data analysis was done using MSTAT-C. Results showed that the herbal extracts significantly were prevented the infection rate of CMV. According to the results in salicylic acid, thyme extract, rosemary extract, and distilled water treatments inoculation rates were 33.3%, 58.3%, 66.6%, and 88.3%, respectively. There wasn’t a statistically significant difference between treatments of salicylic acid and Thyme extract, as well as, spraying with Thyme and Rosemary extracts. Considering the result of this study, it can be concluded that by application of salicylic acid and then Thyme extract, the CMV can be controlled significantly.