The effect of high-intensity interval training on calcitonin gene-dependent peptide in horseshoe muscles of male Wistar rats

Neuromuscular junction and synaptic transmission to skeletal muscle types may be affected by physical exercise. The aim of this study was to investigate the effect of a high-intensity interval training (HIIT) on CGRP gene expression in the horseshoe muscles of male Wistar rats.

In this experimental study, 12 male Wistar rats weighing 200-220 g, with a sleep cycle of 12.12, free access to food and water and an ambient temperature of 25 ° C, were randomly divided into two training and control groups. HIIT training consisted of 6 to 12 repetitions of a 2-minute periodic exercise with a one-minute break between each repetition. The workout was designed for 5 days a week and 6 weeks. The conditions of the control group were similar to the training group but they did not exercise. Finally, 48 hours after the last training session, rats were extracted by standard anesthesia, victim and horseshoe muscle method of all mice, and then Western blot method was used to determine the expression of CGRP protein. Significance level was considered p> 0.05.

Statistical findings from the output of independent t-test for CGRP receptor protein expression values showed that implementation of six weeks of HIIT training protocol caused an increase in CGRP protein expression compared to the control group, which was not statistically significant (p = 0.078).