Protective effect of mixture of Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus holoticus on small intestinal tissue of male poisoned rats lead acetate

One of the most important polluters of the environment is lead acetate. Probiotics are live microbes that are safe, useful and have antioxidant activity for the host. The aim of this research is to investigate the effect of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus heloticus) on changes in the small intestine tissue of male rats exposed to lead acetate.In this experimental study, 21 male Wistar rats weighing 200-250 grams were randomly divided into three groups: intact, treated with lead acetate (10 mg/kg) and treated with lead acetate (10 mg/kg)+ native Iranian probiotic mixture (109 CFU mg/ml) were divided.After five weeks of treatment, the rats were completely anesthetized and the small intestine tissue was collected. After their fixation and processing, 5 micrometer tissue sections were prepared and E&H staining was done. Data were compared with SPSS software, one-way analysis of variance and Tukey's test.Examining the morphological changes of small intestine tissue (inflammation, thickness of muscle layer, height of villi and wound) in the treatment group with probiotics compared to the lead acetate group showed a decrease in tissue damage.According to the findings of this research, the use of a mixture of native Iranian probiotics (Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus heloticus) can have a positive effect on reducing lead acetate-induced damage in the small intestine tissue.