Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model

Mitochondrial dysfunction is one of the possible mechanisms for the progression of heart disease. This study aimed to effect of eight weeks of aerobic training and curcumin supplementation on PGC1- α gene expression and cardiomyocyte mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model.

40 male rats were randomly divided into 5 groups: healthy control, heart myocardial infarction control, aerobic exercise, supplement and exercise + supplement. The training and training+supplement groups were influenced by training on the intelligent electronic animal treadmill for 8 weeks, 5 days a week. At this time, the myocardial infarction control and supplement groups had no exercise program. First, healthy controls were killed. Expression of PGC1-α gene and mitochondrial citrate synthase using real-time PCR method, the obtained data were analyzed using Shapiro-Wilk test, two-way and one-way analysis of variance and Tukey's post hoc test.

Results showed that the expression of PGC1-α and citrate synthase of cardiomyocytes in the groups trained with and without curcumin consumption is significantly higher than the supplemented and myocardial infarction control groups (P=0.001). No significant difference was observed between the two groups of exercise and exercise + supplement. The expression of both genes in the myocardial infarction control group was lower than that of healthy controls (P≤0.05).

Eight weeks of aerobic exercise alone and in combination with curcumin increased the expression of PGC1-α gene and citrate synthase. The combined effect of exercise and curcumin were nonsignificantly greater.