The Antioxidant Effect of N-Acetylcysteine on the Expression of SOD and HOG Genes in Human Dermal Fibroblast in High Glucose State

Wound healing in diabetic patients is delayed, because of oxidative stress. This study aims at investigating the molecular changes in Human Dermal Fibroblasts (HDFs) in a high-glucose state and improving the effect of N-Acetyl-L-Cysteine (NAC) and gene expression of oxidative genes Super-Oxide Dismutase (SOD) and Heme Oxygenase1 (HO1). HDFs were cultured in 5.5, 25, 50, and 75 mM glucose concentrations for 72 hours. Cell proliferation was examined via 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) assays. Oxidative stress markers of SOD and HO1 were quantified with real-time Polymerase Chain Reaction (PCR). The antioxidant effect of NAC on 1 mM was examined to evaluate oxidative markers in the glucose effects on the HDFs. The MTT assay revealed a decline in cell viability in 50 and 75 mM glucose concentrations. mRNA level of SOD and HO1 was upregulated. The antioxidant addition of NAC reduced the inhibitory effect of the high-glucose state on the proliferation of the HDFs. A high-glucose state impairs the in vitro proliferation and migration of HDFs and may, therefore, induce increased oxidative stress and cellular dysfunction. The antioxidant effect of NAC ameliorates the damaging impact of a high-glucose state.