THE EFFECT OF DIALLYL SULFIDE ON LIPOPOLYSACCHARIDE-INDUCED ACUTE KIDNEY DYSFUNCTION IN THE MOUSE

Diallyl sulfide (DAS) is an organosulfur compound derived from garlic with antioxidative and multiple protective effects. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria that is commonly used to create an animal models of Acute Kidney Dysfunction (AKI). LPS can cause renal injury by induction of oxidative stress reactions. According to the role of oxidative stress in the incidence of AKI caused by sepsis, the purpose of this study was to determine the effect of DAS on acute kidney dysfunction due to LPS.

In this experimental study, 32 male C57BL / 6 mice were divided randomly into 4 groups of control, LPS, and two LPS under treatment with DAS (50, and 200 mg/kg) groups. In two DAS treatment groups, oral administration of diallyl sulfide was performed at a single dose and one hour before injection of LPS. AKI was induced by a single dose intraperitoneal injection of 10 mg/kg of LPS. Twenty-four hours after LPS injection, blood samples were collected via heart for BUN and creatinine analysis. Also, homogenization of kidney tissue was prepared to measure oxidative stress markers including malondialdehyde (MDA), nitrite, and catalase.

As compared with LPS group, DAS administered at 200 mg/kg significantly reduced MDA, but reduce in nitrite and increase in catalase activity were not significant. Also, in order to improve kidney function, it significantly reduced serum BUN and creatinine levels as compared with LPS group.

DAS can improve renal dysfunction following LPS, and its beneficial effect is through reducing oxidative stress and possible enhancement of antioxidant defence system.