Study of Morphological Changes in Liver Primary Cell Culture Exposed to Benzo[a]pyrene in Grouper Fish, (Epinephelus coioides)

The liver is one of the most important organs of animals that know as a detoxifying and filtering toxin in the body and the most studies was used the target organ. In peresent study, hepatocytes were isolated from 8 immature grouper fish (equal to the number of male and female). Then, anesthetized fish and the head was separated from the body. The fish wiped with 70% etanol alchol. The liver was removed was cuted with scissors. Finally, the cells digested by collagen type 4. Then, the cells incubated in 30^C and 20% FBS+ITS during two weeks. The doses of pollutant (100 µM, 2*100 µM, 3*100 µM) added to samples, then were studied in 0, 6, 12 and 24 time. The morphological analysis exhibited that the cell death incubated by B[a]P polluted by concentration of 100 µm results in 50% of apoptosis cell death. Our finding from this study showed that using lower dosage of B[a]P during 24h treatment period cause inhibition of proliferation in time and dose dependent manner and higher doasage cause lysis and necrosis.