The effect of HIIT and CGRP inhibition on EGF and EGFR gene expression in the hippocampal tissue of male Wistar rats

Growth factors are small peptide biomolecules that play an important role in increasing autoimmune diseases and are involved in the pathogenesis of many types of cancer. The main purpose of this study is to investigate the effect of 6 weeks of HIIT with inhibition of CGRP on EGF and EGFR gene expression in the hippocampal tissue of male Wistar rats.
In this experimental study, 32 Wistar male laboratory rats with an average weight of 205±15 grams and an age of 8 weeks were randomly divided into 4 groups of 8 series including         1-control (C), 2-inhibition of CGRP (CI), 3-HIIT (TH). ) and               4-inhibition of CGRP+HIIT (CI+TH) were divided. In order to inhibit CGRP in groups 2 and 4, CGRP antibody was used intraperitoneally at a dose of 0.25 mg/kg. The HIIT training protocol was performed for 6 weeks and 5 days per week, which consisted of 10 bouts of intense       2-minute running. on the treadmill and 1 minute of slow running (active rest) was incremental. Finally, the desired tissue was extracted and the expression level of EGF and EGFR genes was evaluated by Real time-PCRΔΔ method and using 2 ΔΔCt formula. To analyze the findings, one-way analysis of variance was used along with Tukey's post hoc test in SPSS software version 26 (p≤0.05).
The results of the present study showed that the levels of EGF in hippocampal tissue in group (TH) were significantly higher than groups (CI) (P=0.001) and (C) (P=0.006). It was also significantly higher in the (CI+TH) group than the (CI) group (P=0.007). EGFR gene expression levels in   (CI+TH) group were significantly higher than (CI) (P=0.04) and (TH) (P=0.01).
Physiological changes caused by HIIT in the hippocampus can improve the functional state of the brain through positive regulation of EGF and EGFR gene expression as an effective non-pharmacological method. Furthermore, HIIT+CGRP inhibition may represent a novel approach through the EGF and EGFR pathways in the hippocampus.