Association of LncRNAHULC Gene rs17144343 G>APolymorphism with Susceptibility to Recurrent Spontaneous Miscarriage in Women of Eastern Azerbaijan, Iran

Women who experience more than two miscarriages suffer from recurrent pregnancy loss(RPL) which involves about 0.8% to 1.4% of couples. The lncRNA HULC gene was first discovered for its significant regulatory function in Hepatocellular carcinoma and then was considered in other diseases including RPL. The present study was performed with aim to investigate the association of G>A single nucleotide polymorphism of rs17144343 in HULC gene with susceptibility to recurrent miscarriages in women population of Eastern Azerbaijan of Iran.

In this case-control study which was performed in 2019-2020, genomic DNAs of 150 patients and 150 healthy individuals were extracted from the peripheral blood samples by salting-out method. Then, genotyping of rs17144343 locus was determined by TETRA-ARMS-PCR assay. Finally, the genotyping data were statistically analyzed using the software package javastat online statistics and SPSS (version 23).

The genotypic distribution of rs17144343 for GA and GG genotypes were respectively 24.7% and 75.3% for cases and 2% and 98% for the control group. The frequency of allele A in controls was 1% and in case individuals was 12.4%. There was a significant relationship between the risk of recurrent pregnancy loss and rs17144343 polymorphism of HULC gene (P<0.001). Also, by considering some clinical and pathological characteristics, it was revealed that there is a significant relationship between genotypes and the familial relationship between the couple's parents (P=0.003).

The results obtained in his study demonstrated that G>A polymorphism of rs17144343 in HULC gene has a significant role in susceptibility to recurrent pregnancy loss, but larger sample studies are required to further verify this finding.