Determination of Total Phenolic and Flavonoid Contents, and Antioxidant Capacity of Methanolic Extract of Tribulus Terrestris at Three Developmental Stages: A Short Report

Tribulus terrestris is one of the medicinal plants. The aim of this study was to determine the amount of total phenol and flavonoid, and antioxidant activity in different developmental stages of the aerial parts of two populations of T. terrestris.

The present laboratory study was conducted in 2021 in two habitats, warm and cold climate, in Ilam Province. The amount of total phenol, the total flavonoid content, and the antioxidant activity of the metanolic extract of the aerial parts of the plants were measured and expressed as IC50 (The half-maximal inhibitory concentration). Data were analyzed using one-way analysis of variance.

The highest and the lowest total phenol and flavonoid contents were obtained respectively in the flowering stage of warmer climate and the stem emergence stage of colder region. The highest and the lowest antioxidant activity were respectively related to the flowering and the stem emergence stages of Mehran and Asmanabad habitats.

The flowering stage of Mehran tropical habitat had the highest content of total phenol, total flavonoid, and antioxidant activity.