ASSOCIATION OF RETN GENE POLYMORPHISM WITH SERUM RESISTIN LEVEL, INSULIN RESISTANCE, AND NON-ALCOHOLIC FATTY LIVER DISEASE IN AN IRANIAN POPULATION

Resistin is an adipokine that is secreted from adipose tissue, causing insulin resistance and fatty liver disease. This study aimed to determine the association of resistin gene rs3745368 single nucleotide polymorphisms (G>A+299) with serum resistin level, insulin resistance index, and non-alcoholic fatty liver disease (NAFLD).

This descriptive study was conducted on 160 people (80 healthy participants and 80 patients with NAFLD) who referred to Bo Ali and Amirul Mominin Hospitals in Tehran in 2022. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine genotype of resistin gene polymorphism rs1862513. Serum resistin and insulin levels were measured using ELISA (Enzyme-linked immunosorbent assay) method, and other parameters were measured by standard methods. Data were analyzed by SPSS statistical software (version20) using independent t test, Mann-Whitney U test, Kruskal-Wallis, ANOVA Spearman’s Correlation Test, and logistic regression test.

In the present study, significant differences were observed among two groups according to the meanlevels of HDL, triglycerides, insulin, resistin, liver enzymes and variables of age, body mass index, systolic blood pressure and HOMA (Homeostatic model assessment) (P<0.05). Significant correlations were observed between resistin levels with body mass index in healthy group and with triglyceride in patients with NAFLD (P<0.05). In the present study, RENT (Resistin) gene polymorphism rs3745368 had no significant relationship with biochemical and anthropometric variables, resistin level, insulin resistance, and non-alcoholic fatty liver (Ps>0.05).

It appears resistin levels are statistically associated with NAFLD, but RENT rs3745368 polymorphism is not statistically associated with resistin level, NAFLD, and insulin resistance.