Effect of Somatic Antigens of Marshallagia Marshalli on Full Thickness Skin Wound Healing in The Mouse

Helminth-induced type 2 immune responses, which are characterized by the T helper 2 cell-associated cytokines interleukin-4 (IL-4) and IL-13, mediate host protection through enhanced tissue repair, the control of inflammation and worm expulsion. The present study was conducted to determine the effect of somatic antigens of nematode Marshallagia marshalli on healing of full-thickness skin wound healing in mice.

In this study, 72 adult mice were divided into 4 groups A, B, C and D. Subgroups A the dose of 40 μg /mice, B the dose of 20 µg / mice, C the alum adjuvant group and D were considered as negative controls. Twenty four hours after made wounds, all mice were treated subcutaneously at the four sides of the wound. The wounds evaluated at days 0,1.3,6,9,12,15,18 and 21 by taken digital photographs. The images were analyzed by Image J software.

After analyzing a significant difference were showed in the percentage of wound contraction, wound healing and the infiltration rate of inflammatory cells in the treatment group with a dose of 20 μg (P ≤ 0.05).

The results of this study indicate that application of somatic antigens of Marshallia marshalla nematode with a dose of 20 μg subcutaneous injection accelerates the healing process of wound healing.